Dr Jez McCole, of Gleadless Medical Centre and Dr Jim Lee, of Nethergreen Surgery, aim to complete the challenge in three days in an effort to raise £2,000 for the charity Hospice Africa.
They will travel from Walney on the west coast to Whitby on the east coast, passing through the Lake District, Yorkshire Dales and the North Yorkshire Moors along the way.
Dr McCole said: ‘There are a lot more doctors using their bikes than you would think. The RCGP have been on a green kick for a while promoting low carbon and cleaner air and the message does seem to be getting through.
‘We chose Hospice Africa as our charity after a good friend worked for them in Uganda. This organisation can being relief from terrible pain to those suffering from AIDs related and other cancers.’