In figures from April 2007, the average full time male medical practitioner earned £84,586 annually, while female medical practitioners earned just £66,318.
Male practice managers are likely to earn on average 24 per cent more than their female counterparts.
The gap was widest among pharmacy managers, where males on average earned 86 per cent more a year than females.
Sharon Holder, national officer for the GMB’s NHS members, said: ‘These figures show that for the top jobs, like doctors, specialists, and managers, there is a wide gender pay gap for women.
‘For all workers in the health sector, the proper implementation of Agenda for Change, to provide fair play to all, is our top priority.’
There was also a wide range of jobs where equal pay had been achieved, she said.
The Trades Union Congress is launching a public sector pay campaign this week, and the GMB hopes to use it as a platform to publicise the discrepancy.
The figures from the study were taken from the Office of National Statistics Annual Survey of Hours and Earnings 2007.
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