NHS Cambridgeshire said this week that it had issued a termination notice ending its contract with Take Care Now (TCN) from 1 December.
In 2008 a GP's father died after being given 10 times the recommended dose of diamorphine by locum Dr Daniel Ubani, hired by TCN.
His death triggered a DoH investigation, expected to conclude that GPs should have greater commissioning duties.
Chris Banks, chief executive of NHS Cambridgeshire, said it had been monitoring TCN and became 'dissatisfied with the progress being made and concerned about TCN's overall performance'.
Co-op Camdoc will replace TCN. The firm has decided not to contest the decision to terminate its contract.
Dr Jim Kennedy, TCN medical director, said: 'Despite many months of discussion with the PCT, we couldn't agree on a number of issues relating to the out-of-hours needs of patients.'